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The use of environmental RNA (eRNA) is starting to emerge as a method for addressing questions that eDNA cannot answer. eRNA could be a useful tool in determining if an organism is alive or dead. The goal of this project was to investigate eRNA preservation and extraction methods for the detection of the quagga mussel (QM) 16S and 18S transcripts. eRNA samples were collected at Canyon Reservoir, AZ, preserved three different ways, and three different RNA extraction kits were used. RT-qPCR was performed on the samples for both the 16S and 18S transcripts. It was possible to detect the 18S transcript with all three-preservation methods and the three extraction methods. The 16S transcript was not detected when preserved with the RNAlater preservative and with the Zymo Water and Monarch Kits. The project showed that it is possible to extract viable eRNA from water samples. 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Data supports report ST-21045-2024-02. Data files contain a read me explaining contents. Within this zip file are three files containing data from the research project. Files within each of the three primary folders providing data for experiments at Glendo, the Denver Federal Center, and A Canal near Klamath falls. In general the data files contain information from ground penetrating radar, slab impulse response, impact echo, and infrared camera. Data has been checked and peer reviewed according to Reclamation guidelines. Data was collected using the methods provided in the report and generally following industry standard methods (ASTM, etc.). The report discusses the data and interpretation methods.", "itemRecordStatusId":1, "isModeled":false, "hasProfile":false, "disclaimer":"The findings and conclusions of this work are those of the author(s) and do not necessarily represent the views of the Bureau of Reclamation.", "sourceCode":null, "locationSourceCode":null, "externalDataUrl":null, "parameterSourceCode":null, "modelNameSourceCode":null, "temporalStartDate":null, "temporalEndDate":null, "spatialShortDescription":null, "publicationAuthor":"Evan J. Lindenbach, Sarah L. 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Voids could lead to hydraulic jacking, ground instability, and a roofed path for internal erosion processes. Any and all of these conditions could result in a failure, as seen recently at the Fort Laramie tunnel or the Oroville Dam spillway. This report is intended to provide a high-level overview of the topics as they relate directly to the problem of void formation near spillways, conduits, canals, tunnels, and siphons. A series of questions aimed at guiding the investigator towards structures (inventory-wide) and areas (structure-specific) most likely to have voids is provided to streamline detection efficiency. 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There are a variety of\r\ndifferent mechanisms that can lead to the formation of voids adjacent to dam structures (U.S. Bureau of Reclamation, 2019).\r\nDetermining the appropriate action to be taken after a void has been detected involves the careful consideration of many different\r\nfactors. Selection of the appropriate method is always site specific. Designers must consider factors such as the type of materials used\r\nin the original design as well as common design / construction practices at the time of construction. Some other important factors\r\ninclude downstream impacts, extent and location of damage, size and shape of conduit or spillway adjacent to the void, type of\r\nmaterial being eroded, size of dam, and cost of repair. This report details each of the different repair, renovation, and replacement\r\ntechniques for structures associated with void formation, and provides guidance on the appropriate action to be taken. Additionally,\r\nthe report provides the results of laboratory testing on permeable low density cellular concrete and foam transported sand as\r\ninnovative methods to repair voids.", "itemRecordStatusId":1, "isModeled":false, "hasProfile":false, "disclaimer":"The findings and conclusions of this work are those of the author(s) and do not necessarily represent the views of the Bureau of Reclamation.", "sourceCode":null, "locationSourceCode":null, "externalDataUrl":null, "parameterSourceCode":null, "modelNameSourceCode":null, "temporalStartDate":null, "temporalEndDate":null, "spatialShortDescription":null, "publicationAuthor":"Evan J. 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The data files contain a read me describing the contents. Within this zip file are two csv files for the data supporting the void repair portion of this project. This includes data from the PLDCC samples and from the foamed transported sand samples. In general, the data presents the results from laboratory testing of permeable low density cellular concrete and foam transported sand. Methods are discussed in the report but generally followed industry standard methods (ASTM) or typical laboratory procedures. 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Three current state-of the-art are adopted: (a) multi-dimensional flow models; (b) fish track models; and (c) knowledge of fish behaviors in response to flow features. Two flow models are updated to provide the flow variables to which juvenile fish respond: 2-D hydraulic model SRH-2D and a 3-D computational fluid dynamics (CFD) model. Two fish track tools, one in the Eulerian framework and the other in the Lagrangian framework, are developed so that fish entrainment at diversion channel junctures may be evaluated. A third sophisticated tool ELAM, a fish track model developed by Army Corps of Engineers (USACE), is further adopted through a collaboration with USACE. These flow and fish track tools have been applied to several diversion junctures, including Reclamation projects; valuable knowledge has been gained in understanding the ways to alter the flow features to increase or decrease fish entrainment. 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